| Expression pattern: |
UP |
| Associated gene: |
HMGA2, Ago2, Ki-67, PCNA, ASCT2, GLS1 |
| Associated microRNA: |
miR-3064-5p |
| Biological function: |
circNFIX promotes HCC cell proliferation, migration, invasion, tumor growth and glutaminolysis; circNFIX knockdown represses these malignant phenotypes. |
| Molecular mechanism: |
circNFIX acts as a competing endogenous RNA that sponges miR-3064-5p, thereby releasing/enhancing HMGA2 expression and promoting glutaminolysis and HCC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glutaminolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circNFIX is associated with poor survival and may be a biomarker and therapeutic target for HCC. |
| Description: |
circNFIX is up-regulated in HCC tissues and cell lines, and high circNFIX expression is associated with poor overall survival. Functionally, circNFIX promotes HCC proliferation, migration, invasion, xenograft tumor growth and glutaminolysis. Mechanistically, circNFIX acts as a ceRNA for miR-3064-5p to enhance HMGA2 expression, supporting the circNFIX/miR-3064-5p/HMGA2 axis as a potential therapeutic target. |
| Confidence score: |
0.8314 |