| Expression pattern: |
UP |
| Associated gene: |
RAP1B, ESRP1 |
| Associated microRNA: |
miR-149 |
| Biological function: |
promotes proliferation, migration, invasion, epithelial-mesenchymal transition and glycolysis; promotes tumor growth and metastasis |
| Molecular mechanism: |
ceRNA sponge of miR-149 to increase RAP1B; biogenesis regulated by ESRP1 via binding flanking introns; positive feedback involving lactate-driven ESRP1 lactylation/stabilization |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); glycolysis (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
high circPHGDH expression was related to large tumor size, high T stage and distant metastasis |
| Description: |
circPHGDH is up-regulated in prostate cancer and promotes proliferation, migration, invasion, EMT, glycolysis, and in vivo tumor growth/metastasis. It functions as a ceRNA sponging miR-149 to upregulate RAP1B, which activates PI3K/AKT signaling. ESRP1 promotes circPHGDH biogenesis, and lactate-driven ESRP1 lactylation stabilizes ESRP1 to form a positive feedback loop. |
| Confidence score: |
0.8464 |