| Expression pattern: |
UP |
| Associated gene: |
SEPT10, METTL3 |
| Associated microRNA: |
- |
| Biological function: |
Exosomal circCLIP1 promotes PM2.5-induced mucus secretion in recipient HBE cells and contractility of sensitive HBSMCs. |
| Molecular mechanism: |
PM2.5 increases METTL3-mediated m6A modification of circCLIP1, upregulating exosomal circCLIP1; exosome-transmitted circCLIP1 is internalized by recipient cells and enhances SEPT10 expression to induce mucus hypersecretion and smooth muscle contractility. |
| Biological pathway or process: |
m6A modification (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; MeRIP / MeRIP-seq; Transfection; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circCLIP1 was associated with acute PM2.5 exposure in paired human plasma samples and was proposed as a potential biomarker for PM2.5-related adverse effects. |
| Description: |
This study identifies exosomal circCLIP1, derived from CLIP1, as an up-regulated circRNA after PM2.5 exposure. METTL3-mediated m6A modification increases circCLIP1 expression and exosomal abundance, and exosome-transmitted circCLIP1 promotes mucus hypersecretion in HBE cells and contractility in sensitive HBSMCs by enhancing SEPT10. Human plasma data suggest exosomal circCLIP1 may serve as a biomarker of PM2.5-related adverse effects. |
| Confidence score: |
0.8414 |