| Expression pattern: |
UP |
| Associated gene: |
PHIP, ACTN4, AGO2 |
| Associated microRNA: |
miR-142-5p |
| Biological function: |
promotes OSCC cell migration, invasion, and proliferation; enhances tumor xenograft growth |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-142-5p to increase PHIP and ACTN4 expression and activate AKT/mTOR signaling. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); migration (promotes); invasion (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Associated with tumor metastasis, TNM stage, and HPV infection status. |
| Description: |
circPHIP (hsa_circ_0001615), derived from PHIP, is up-regulated in OSCC tissues and cell lines and mainly localizes to the cytoplasm. It promotes OSCC proliferation, migration/invasion and xenograft growth by sponging miR-142-5p, thereby increasing PHIP and ACTN4 expression and activating PI3K/AKT/mTOR signaling. |
| Confidence score: |
0.8072 |