circRNA basic information
circBase ID: hsa_circ_0000605
Name: hsa_circ_SLTM
Synonym: Circular RNA SLTM / circSLTM
Host Gene: SLTM
Genomic location(hg19): chr15:59204761-59209198:-
Genomic location(hg38): chr15:58912562-58916999:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005178
MONDO name: osteoarthritis
Disease details: Osteoarthritis / OA
Disease DO ID:
8398
Disease MeSH ID:
D010003
Disease NCIt ID:
C3293
Disease ICD11 ID:
558562409
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

cartilage tissue; knee tissues; knee joints

Cell lines:

HEK293

In vivo animal model:

other disease animal model

circRNA-disease information
Expression pattern:
UP
Associated gene: HMGB2, Ago2, MMP-1, MMP-13, cleaved caspase-3, cytochrome C, p-NF-kappaB, IL-6, IL-8, TNF-alpha
Associated microRNA: miR-421
Biological function: circSLTM promotes OA chondrocyte apoptosis and inflammation, reduces chondrocyte viability, increases LDH release and ROS production, and aggravates cartilage destruction; circSLTM knockdown ameliorates apoptosis, inflammation, and cartilage damage in OA models.
Molecular mechanism: circSLTM acts as a competing endogenous RNA for miR-421, thereby regulating HMGB2 expression and promoting p-NF-kappaB-related inflammatory signaling and apoptosis in OA chondrocytes.
Biological pathway or process:

apoptosis (promotes); inflammation (promotes); NF-kappaB (promotes); proliferation (inhibits); ceRNA regulation (other); other pathway/process (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); TUNEL

Clinical significance:

circSLTM may serve as a potential molecular target for OA therapy.

Description:

circSLTM is up-regulated in IL-1beta-induced OA chondrocytes and in OA rat cartilage/knee tissue models. It functions as a miR-421-competing endogenous RNA to regulate HMGB2, thereby promoting NF-kappaB-related inflammation, ROS production, apoptosis, and cartilage damage; knockdown of circSLTM alleviates OA-like injury in vitro and in vivo.

Confidence score:

0.6279

Other information
Title:

Circular RNA SLTM as a miR-421-competing endogenous RNA to mediate HMGB2 expression stimulates apoptosis and inflammation in arthritic chondrocytes.

Journal: Journal of biochemical and molecular toxicology
Published: 2023
PubMed ID: 36935520
Study type:

biological research

Data availability: available from the corresponding author upon reasonable request
Code availability: -