| Expression pattern: |
UP |
| Associated gene: |
IL32, MS4A4A, FOSL1, MAGEC1 |
| Associated microRNA: |
Hsa-miR-409-3p, hsa-miR-579-5p, hsa-miR-1238-3p |
| Biological function: |
Upregulated in MDS patients with U2AF1 mutations; knockdown in U2AF1-mutated K562 cells led to significant gene expression changes, including downregulation of IL32 and upregulation of MS4A4A, FOSL1 and MAGEC1. |
| Molecular mechanism: |
Predicted miRNA interaction and downstream gene expression regulation after circRNA knockdown; the article also relates upregulation to altered circRNA biogenesis in the presence of U2AF1 mutations. |
| Biological pathway or process: |
ceRNA regulation (other); mRNA stability (other); other pathway/process (other) |
| Detected method: |
S
|
| Validation methods: |
RNA-seq; Clinical Sample Validation; Bioinformatics Analysis; Transfection; RT-qPCR |
| Clinical significance: |
Potential biomarker and therapeutic target investigation in U2AF1-mutated MDS. |
| Description: |
circMAN1A2 was one of seven circRNAs upregulated in MDS patients with U2AF1 mutations and independently confirmed in cohort 2. Functional knockdown in U2AF1-mutated K562 cells altered cancer-panel gene expression, especially IL32, MS4A4A, FOSL1 and MAGEC1, suggesting a potential role in U2AF1-mutated MDS biology. |
| Confidence score: |
0.4997 |