circRNA basic information
circBase ID: hsa_circ_0056856
Name: hsa_circ_ITGB6
Synonym: circITGB6
Host Gene: ITGB6
Genomic location(hg19): chr2:160980301-160983112:-
Genomic location(hg38): chr2:160123790-160126601:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tumor tissues; lung tumor tissues; metastatic liver lesions; metastatic lung tissues
Cell lines:

MCF10A; A549; HCT116; HT29; SW620; MCF7; SW480; HCC827; Capan-2; Py2T; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF2BP3, PDPN mRNA
Associated microRNA: -
Biological function: promotes EMT process and tumor metastasis; promotes cell migration
Molecular mechanism: circITGB6 directly interacts with IGF2BP3 and enhances IGF2BP3-mediated PDPN mRNA stability
Biological pathway or process:

EMT (promotes); metastasis (promotes); migration (promotes); mRNA stability (promotes)
Detected method:
Q
B
S
Validation methods:

circRNA-seq; Bioinformatics Analysis; RT-qPCR; Northern Blot; divergent primers PCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Actinomycin D / DRB Stability Assay; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis
Clinical significance:

Higher circITGB6 expression is associated with lymph node metastasis and worse prognosis of CRC patients; circITGB6 is positively correlated with PDPN in CRC and lung cancer samples
Description:

circITGB6 (hsa_circ_0056856), a TGFbeta-induced circRNA from ITGB6, is up-regulated in metastatic cancer samples and promotes EMT, migration, and metastasis. It directly binds the RBP IGF2BP3 to enhance PDPN mRNA stability, forming a pro-metastatic circITGB6/IGF2BP3/PDPN axis associated with poor prognosis in CRC.
Confidence score:

0.8917
Other information
Title:

A circular RNA activated by TGFbeta promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability.

Journal: Nature communications
Published: 2023
PubMed ID: 37898647
Study type:

combined biological and clinical study
Data availability: GSE165576; PXD043341
Code availability: -