| Expression pattern: |
DN |
| Associated gene: |
GLS, Ago2 |
| Associated microRNA: |
miR-125a-5p |
| Biological function: |
Contributes to teriparatide-promoted osteogenic differentiation of hMSCs; promotes glutamine metabolism and osteogenesis. |
| Molecular mechanism: |
ceRNA mechanism: circFNDC3B sponges miR-125a-5p, relieving repression of GLS and enhancing glutamine metabolism to promote osteogenesis under teriparatide treatment. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (other); glutaminolysis (promotes); osteogenesis (other pathway/process) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Clinical Sample Validation |
| Clinical significance: |
circFNDC3B could serve as a diagnostic biomarker for osteogenesis (in OP context). |
| Description: |
In primary hMSCs from osteoporosis patients, circFNDC3B is down-regulated. circFNDC3B promotes teriparatide-induced osteogenic differentiation by sponging miR-125a-5p, thereby relieving inhibition of GLS and supporting glutamine metabolism required for osteogenesis. |
| Confidence score: |
0.6947 |