circRNA basic information
circBase ID: hsa_circ_0002938
Name: hsa_circ_CRIM1
Synonym: -
Host Gene: CRIM1
Genomic location(hg19): chr2:36726361-36749456:+
Genomic location(hg38): chr2:36499218-36522313:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma / ESCC
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Cancer and matched paracarcinoma tissue specimens; ESCC tissues; adjacent normal tissues
Cell lines:

TE-1; KYSE30; KYSE410; KYSE150; Het-1A
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TCF12, AGO2
Associated microRNA: miR-342-3p
Biological function: Promotes ESCC cell proliferation, colony formation, migration, invasion, and EMT; knockdown suppresses malignant progression.
Molecular mechanism: circCRIM1 acts as a ceRNA sponge for miR-342-3p, relieving repression of TCF12 and promoting EMT in ESCC cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

High circCRIM1 expression is positively correlated with TNM stage, tumor invasion range, and lymph node metastasis; circCRIM1 may serve as a predictive biomarker and therapeutic target for ESCC.
Description:

hsa_circ_0002938, also named circCRIM1, is an upregulated onco-circRNA in ESCC derived from CRIM1. It promotes ESCC cell proliferation, colony formation, migration, invasion, and EMT by acting as a ceRNA for miR-342-3p to increase TCF12 expression. Its high expression correlates with advanced TNM stage, tumor invasion range, and lymph node metastasis, suggesting biomarker and therapeutic potential.
Confidence score:

0.8351
Other information
Title:

Circular RNA hsa_circ_0002938 (circCRIM1) promotes the progression of esophageal squamous cell carcinoma by upregulating transcription factor 12.

Journal: Neoplasma
Published: 2023
PubMed ID: 36916930
Study type:

combined biological and clinical study
Data availability: -
Code availability: -