| Expression pattern: |
UP |
| Associated gene: |
RBBP4, HSPB8 |
| Associated microRNA: |
miR-1-3p |
| Biological function: |
Promotes CRC cell proliferation and invasion, accelerates xenograft tumor growth, and promotes micro-angiogenesis. |
| Molecular mechanism: |
CircAGO2 acts as a ceRNA sponge for miR-1-3p to upregulate RBBP4, which inhibits HSPB8 transcription through histone deacetylation/H3K27ac regulation at the HSPB8 promoter. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Clinical Sample Validation; Transfection; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IF (Immunofluorescence); Luciferase Reporter Assay; RNA Pull-Down; Western Blot; ChIP / ChIP-seq; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
High circAGO2 expression is associated with advanced TNM stage and poorer overall survival in CRC patients. |
| Description: |
CircAGO2 is upregulated in colorectal cancer tissues and cell lines and is associated with advanced TNM stage and poorer overall survival. Functionally, circAGO2 promotes CRC cell proliferation, invasion, xenograft tumor growth, and micro-angiogenesis. Mechanistically, circAGO2 sponges miR-1-3p to increase RBBP4 expression, leading to reduced HSPB8 transcription through H3K27ac-related histone deacetylation. |
| Confidence score: |
0.7941 |