| Expression pattern: |
UP |
| Associated gene: |
SIRT1, CPA4, PCNA, MMP9, E-cadherin, Vimentin, Ago2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes glioma cell viability, migration, invasion, glycolysis and xenograft tumor growth; knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
circ0082374 acts as a ceRNA sponge for miR-326 to promote SIRT1 expression in glioma cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ0082374 is associated with advanced stage, lower overall survival, tumor size and WHO stage, and may predict poor outcome of glioma patients. |
| Description: |
circ0082374 is up-regulated in glioma tissues and cell lines, and high expression is associated with advanced stage and poor overall survival. Functionally, circ0082374 promotes glioma cell viability, migration, invasion, glycolysis and xenograft tumor growth. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-326 to increase SIRT1 expression. |
| Confidence score: |
0.8562 |