| Expression pattern: |
UP |
| Associated gene: |
ASPM, CENPW, KIF14, NEK2, POLQ, TOP2A, DBF4, ERCC6L, E2F7, GPC3, MMS22L, Ago2 |
| Associated microRNA: |
miR-378a-3p, miR-378c |
| Biological function: |
promotes proliferation; promotes cell cycle progression; promotes cell transformation (anchorage-independent growth); promotes migration; promotes invasion |
| Molecular mechanism: |
Acts as a miRNA sponge/ceRNA for miR-378a-3p to upregulate ASPM; interacts with Ago2/RISC; directly binds miR-378a-3p (pull-down); validated by luciferase reporters with WT/Mut binding sites. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (other); ceRNA regulation (promotes); Wnt/beta-catenin (other) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Microarray; RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Cell Cycle Assay; Colony Formation Assay; CCK8; Transwell Assay; Wound Healing Assay; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circGPC3 expression is significantly associated with high tumor grade; circGPC3 has potential as a non-invasive prognostic biomarker with plasma levels highest in higher-grade tumors and lowest in healthy individuals; ROC/logistic regression indicates ability to distinguish tumor vs non-tumorous tissue and tumor grades. |
| Description: |
circGPC3 is up-regulated in HCC tumors and is linked to higher tumor grade. It promotes proliferation, cell-cycle progression, transformation, migration and invasion, and functions as a ceRNA by sponging miR-378a-3p (and predicted miR-378c) to upregulate ASPM, supported by luciferase, Ago2-RIP and pull-down assays. |
| Confidence score: |
0.8778 |