circRNA basic information
circBase ID: hsa_circ_0069094
Name: -
Synonym: circ_0069094
Host Gene: -
Genomic location(hg19): chr4:6698619-6698897:+
Genomic location(hg38): chr4:6696892-6697170:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; paracancerous normal breast tissues
Cell lines:

MCF-7; MDA-MB-231; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HMGA1
Associated microRNA: miR-661
Biological function: Hsa_circ_0069094 promotes breast cancer cell glycolysis, proliferation, migration, invasion and tumor growth, while inhibiting cell apoptosis and G1 cell-cycle arrest.
Molecular mechanism: Hsa_circ_0069094 functions as a miR-661 sponge to regulate HMGA1 expression, thereby promoting breast cancer progression and glycolysis.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Survival Analysis; Cohort Study; Bioinformatics Analysis
Clinical significance:

High hsa_circ_0069094 expression was correlated with lymph node metastasis and low survival time in breast cancer patients.
Description:

Hsa_circ_0069094 is up-regulated in breast cancer tissues and cells and high expression is associated with poor survival and lymph node metastasis. Functional experiments show that hsa_circ_0069094 promotes proliferation, migration, invasion, glycolysis and tumor growth while suppressing apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-661 to regulate HMGA1.
Confidence score:

0.8202
Other information
Title:

Hsa_circ_0069094 knockdown inhibits cell proliferation, migration, invasion and glycolysis, while induces cell apoptosis by miR-661/HMGA1 axis in breast cancer.

Journal: Anti-cancer drugs
Published: 2021
PubMed ID: 33929992
Study type:

combined biological and clinical study
Data availability: Supplementary Table 1, Supplemental digital content 1, http://links.lww.com/ACD/A383
Code availability: -