circRNA basic information
circBase ID: hsa_circ_0000591
Name: -
Synonym: hsa_circRNA_101488
Host Gene: -
Genomic location(hg19): chr15:41961025-41962156:+
Genomic location(hg38): chr15:41668827-41669958:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS samples; matching non-tumour samples; xenograft tumours
Cell lines:

HOS; MG63; U2OS
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HK2, GLUT1, LDHA
Associated microRNA: miR-194-5p
Biological function: Promotes OS cell viability, proliferation, colony formation, invasion, glycolysis, and in vivo tumour growth; inhibits apoptosis.
Molecular mechanism: circ_0000591 functions as a ceRNA/molecular sponge for miR-194-5p, thereby upregulating HK2 expression and promoting OS glycolysis and progression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Cohort Study
Clinical significance:

Higher circ_0000591 expression levels were associated with shorter overall survival in OS patients.
Description:

hsa_circ_0000591/circ_0000591 is up-regulated in osteosarcoma tissues and cell lines, and high expression is associated with shorter overall survival. Functionally, it promotes OS proliferation, invasion, glycolysis, and xenograft growth while suppressing apoptosis. Mechanistically, circ_0000591 acts as a cytoplasmic miR-194-5p sponge to elevate HK2 expression, thereby driving OS progression and glycolysis.
Confidence score:

0.8497
Other information
Title:

Hsa_circ_0000591 drives osteosarcoma glycolysis and progression by sequestering miR-194-5p and elevating HK2 expression.

Journal: Clinical and experimental pharmacology & physiology
Published: 2023
PubMed ID: 36809521
Study type:

combined biological and clinical study
Data availability: GSE96964; available from the corresponding author on reasonable request
Code availability: -