circRNA basic information
circBase ID: -
Name: hsa_circ_ADSS
Synonym: -
Host Gene: ADSS
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; tumour tissues; normal tissues
Cell lines:

Huh7; SNU423; MHCC97H; HCCLM3; THLE-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TOP2A
Associated microRNA: miR-431-5p
Biological function: Promotes HCC cell proliferation, cell cycle progression, migration, invasion, EMT, and tumour growth in vivo.
Molecular mechanism: circADSS acts as a sponge for miR-431-5p and regulates TOP2A through the miR-431-5p/TOP2A axis.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNase R Treatment; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Transwell Assay; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

circADSS is up-regulated in HCC tumour tissues and cell lines. Functionally, circADSS promotes HCC cell proliferation, cell cycle progression, migration, invasion, EMT, and xenograft tumour growth. Mechanistically, it acts through a circADSS/miR-431-5p/TOP2A regulatory axis.
Confidence score:

0.8169
Other information
Title:

CircADSS contributes to hepatocellular carcinoma development by regulating miR-431-5p/TOP2A.

Journal: Clinical and experimental pharmacology & physiology
Published: 2023
PubMed ID: 36786410
Study type:

combined biological and clinical study
Data availability: GSE164803
Code availability: -