circRNA basic information
circBase ID: hsa_circ_0001947
Name: hsa_circ_AFF2
Synonym: -
Host Gene: AFF2
Genomic location(hg19): chrX:147743428-147744289:+
Genomic location(hg38): chrX:148661907-148662768:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008383
MONDO name: rheumatoid arthritis
Disease details: rheumatoid arthritis / RA
Disease DO ID:
 7148
Disease MeSH ID:
 D001172
Disease NCIt ID:
 C2884
Disease ICD11 ID:
 576319925
Disease OMIM ID:
 180300
Species: Human
Species details: Homo sapiens
Tissue specimen:

synovial tissues
Cell lines:

RA-FLSs; normal FLSs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3
Associated microRNA: miR-671-5p
Biological function: promotes proliferation, invasion, migration and inflammatory response; inhibits apoptosis in RA-FLSs
Molecular mechanism: ceRNA mechanism: circ_0001947 sponges miR-671-5p to elevate STAT3 expression
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); invasion (promotes); migration (promotes); inflammation (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; ELISA; Western Blot; Clinical Sample Validation
Clinical significance:

-
Description:

circ_0001947 (circAFF2) is up-regulated in RA synovial tissues and RA-FLSs, mainly localizes to the cytoplasm, and promotes RA-FLS proliferation, invasion, migration and inflammatory cytokine production while inhibiting apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-671-5p to increase STAT3 expression (circ_0001947/miR-671-5p/STAT3 axis).
Confidence score:

0.7229
Other information
Title:

Circ_0001947 promotes cell proliferation, invasion, migration and inflammation and inhibits apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes through miR-671-5p/STAT3 axis.

Journal: Journal of orthopaedic surgery and research
Published: 2022
PubMed ID: 35093109
Study type:

combined biological and clinical study
Data availability: -
Code availability: -