| Expression pattern: |
UP |
| Associated gene: |
TGFbetaR2, phosph-SMAD3, Ago2 |
| Associated microRNA: |
miR-370-3p / miR-370 |
| Biological function: |
inhibits proliferation, migration and tube formation of ox-LDL-exposed HAECs |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-370 to promote TGFbetaR2 and downstream phosph-SMAD3 expression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); angiogenesis (inhibits); TGF-beta/SMAD (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Sanger Sequencing; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; Clinical Sample Validation; ROC Analysis; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Upregulated in plasma EVs of patients with cerebral atherosclerosis and shows diagnostic/prognostic potential (ROC; higher expression associated with more end events). |
| Description: |
In ox-LDL-treated human aorta endothelial cells and in plasma extracellular vesicles from cerebral atherosclerosis patients, circ_0003204 is upregulated. It mainly localizes to the cytoplasm and suppresses endothelial proliferation, migration, and tube formation by acting as a ceRNA for miR-370-3p, thereby increasing TGFbetaR2 and downstream phospho-SMAD3 signaling. Clinically, higher EV circ_0003204 shows diagnostic value and is associated with worse event-free outcome in cerebral atherosclerosis patients. |
| Confidence score: |
0.8766 |