circRNA basic information
circBase ID: hsa_circ_0000467
Name: hsa_circ_SKA3
Synonym: circSKA3 / circ-SKA3
Host Gene: SKA3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm; membrane
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human breast cancer specimen; adjacent benign tissues; tumor xenografts; tumor tissues; muscle tissues
Cell lines:

MCF-10A; HaCaT; 293T; Jurkat; MCF10A; MCF-7; MDA-MB-468; MDA-MB-231; HTB126; SK-BR-3; H460; LnCap; Du145; PC3; SNU449; HepG2; CI3K; A549; HeLa
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Tks5, integrin b1, Itgb1, ERK, MMP2, MMP11, Cdc42
Associated microRNA: -
Biological function: Promotes breast cancer cell migration, invasion, proliferation, tumor progression, tumor growth, tumor cell invasion, and invadopodium formation.
Molecular mechanism: circSKA3 binds and bridges Tks5 and integrin b1/Itgb1, recruits Tks5 to the cell membrane, promotes Tks5 phosphorylation, and induces circSKA3-Itgb1-Tks5 complex formation underlying invadopodium formation.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Northern Blot; RNase R Treatment; Sanger Sequencing; Transfection; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

circSKA3 is highly expressed in human breast cancer patient samples and positively correlated with disease staging.
Description:

circSKA3 is up-regulated in human breast cancer cells and tissues and is positively correlated with disease stage. It promotes breast cancer migration, invasion, proliferation, tumor progression, and invadopodium formation by binding and bridging Itgb1/integrin beta1 and Tks5 at the membrane/invadopodia. In xenograft models, circSKA3 overexpression increases tumor growth and invasion, whereas circSKA3 silencing reduces invadopodia and improves mouse survival.
Confidence score:

0.8395
Other information
Title:

The Circular RNA circSKA3 Binds Integrin beta1 to Induce Invadopodium Formation Enhancing Breast Cancer Invasion.

Journal: Molecular therapy : the journal of the American Society of Gene Therapy
Published: 2020
PubMed ID: 32229309
Study type:

combined biological and clinical study
Data availability: Supplemental Information can be found online at https://doi.org/10. 1016/j.ymthe.2020.03.002.
Code availability: -