circRNA basic information
circBase ID: hsa_circ_0084188
Name: hsa_circ_KIAA0146
Synonym: circ_0084188
Host Gene: KIAA0146
Genomic location(hg19): chr8:48192449-48206619:+
Genomic location(hg38): chr8:47279861-47294030:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; paired adjacent non-tumor tissues; tumor tissues
Cell lines:

HCT116; SW620; NCM460
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KIF20A, KIAA0146 mRNA
Associated microRNA: miR-769-5p
Biological function: promotes CRC cell proliferation, migration, invasion, EMT and xenograft tumor growth; inhibits apoptosis
Molecular mechanism: circ_0084188 acts as a ceRNA sponge for miR-769-5p to upregulate KIF20A expression in CRC
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

circ_0084188 expression was associated with tumor size, TNM stage, and overall survival; high circ_0084188 expression predicted shorter overall survival
Description:

circ_0084188 is up-regulated in colorectal cancer tissues and cell lines, and high expression is associated with larger tumor size, advanced TNM stage, and poorer overall survival. Functionally, circ_0084188 promotes CRC proliferation, migration, invasion, EMT and xenograft tumor growth while suppressing apoptosis. Mechanistically, it sponges miR-769-5p to increase KIF20A expression, forming a circ_0084188/miR-769-5p/KIF20A regulatory axis.
Confidence score:

0.8712
Other information
Title:

Circ_0084188 Regulates the progression of colorectal cancer through the miR-769-5p/KIF20A axis.

Journal: Biochemical genetics
Published: 2023
PubMed ID: 36763221
Study type:

combined biological and clinical study
Data availability: Supplementary material available at https:// doi. org/ 10. 1007/ s10528-023-10339-3
Code availability: -