| Expression pattern: |
UP |
| Associated gene: |
Notch1, col1alpha1, alpha-SMA, ASPH, cyclin D1, Bax, Bcl-2, c-Myc |
| Associated microRNA: |
miR-139-5p |
| Biological function: |
Promotes HSC activation and proliferation and inhibits apoptosis in LX-2 cells; associated with increasing disease severity in HC and HCC patient serum and higher expression in HCC tissues. |
| Molecular mechanism: |
circASPH functions as a ceRNA/miRNA sponge that binds miR-139-5p and promotes Notch1 expression. |
| Biological pathway or process: |
Notch (promotes); proliferation (promotes); apoptosis (inhibits); fibrosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; TUNEL; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circASPH may have clinical transformation value and could serve as a diagnostic and prognostic marker for HF; serum levels increase with disease aggravation in HC and HCC patients. |
| Description: |
Human hsa_circ_0009158 is described as a homologous circASPH and is up-regulated in HC/HCC serum, HCC cancer tissues, and TGF-beta1-treated LX-2 cells. Functionally, circASPH promotes HSC activation and proliferation and inhibits apoptosis, likely through the miR-139-5p/Notch1 axis, suggesting potential diagnostic and therapeutic relevance in liver fibrosis-related disease. |
| Confidence score: |
0.8206 |