| Expression pattern: |
UP |
| Associated gene: |
ACVR2B, AGO2 |
| Associated microRNA: |
miR-18a-5p |
| Biological function: |
contributes to OGD/R-induced HBMEC injury; silencing attenuates cell viability inhibition, cytotoxicity, apoptosis, and inflammation |
| Molecular mechanism: |
circ_0000566 acts as a sponge of miR-18a-5p and regulates ACVR2B expression to mediate OGD/R-induced HBMEC injury |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
potential target for the treatment of ischemic stroke |
| Description: |
circ_0000566 is up-regulated in OGD/R-treated human brain microvascular endothelial cells and promotes OGD/R-induced endothelial cell injury. Mechanistically, circ_0000566 sponges miR-18a-5p to regulate ACVR2B expression, thereby contributing to reduced cell viability and increased apoptosis and inflammation in the in vitro ischemic stroke injury model. |
| Confidence score: |
0.7615 |