| Expression pattern: |
UP |
| Associated gene: |
HIF‐1alpha, METTL3, Ago2 |
| Associated microRNA: |
miR‐140‐3p, miR‐182 |
| Biological function: |
promotes proliferation, migration, and invasion; inhibits apoptosis; promotes aerobic glycolysis |
| Molecular mechanism: |
METTL3-dependent m6A modification increases circIST1 stability; circIST1 acts as a ceRNA sponging miR-140-3p and miR-182 to upregulate HIF-1alpha, activating HIF-1alpha-driven glycolysis |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); m6A modification (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; MeRIP / MeRIP-seq |
| Clinical significance: |
High circIST1 expression is associated with poor prognosis, reduced overall and disease-free survival, and advanced tumor stage in HCC. |
| Description: |
circIST1 is up-regulated in hepatocellular carcinoma and predicts poorer survival. METTL3-dependent m6A modification stabilizes circIST1, which mainly localizes to the cytoplasm and promotes HCC proliferation/migration/invasion while inhibiting apoptosis. Mechanistically, circIST1 acts as a ceRNA to sponge miR-140-3p and miR-182, derepressing HIF-1alpha and enhancing HIF-1alpha-driven aerobic glycolysis. |
| Confidence score: |
0.8793 |