| Expression pattern: |
UP |
| Associated gene: |
SP1 mRNA, SP1, AGO2, E-cadherin, N-cadherin, vimentin, snail |
| Associated microRNA: |
miR-136 |
| Biological function: |
Promotes gastric cancer cell proliferation, migration, invasion/metastasis and in vivo tumor growth; acts as an oncogenic circRNA and potential therapeutic target. |
| Molecular mechanism: |
circ-0000979 functions as a cytoplasmic ceRNA that sponges miR-136, reduces miR-136 activity/expression, and modulates SP1 mRNA/protein expression, thereby promoting gastric cancer progression and EMT-related marker changes. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-0000979 expression is associated with poorer prognosis and significantly correlated with GC staging, grade, and lymph node metastasis; circ-0000979 might be a predictive marker and RNA therapeutic target for gastric cancer. |
| Description: |
circ-0000979 is up-regulated in gastric cancer tissues and cell lines, and high expression is associated with poorer prognosis and clinicopathological progression. Mechanistically, cytoplasmic circ-0000979 promotes GC proliferation, migration, invasion/metastasis and xenograft tumor growth by sponging miR-136 and regulating SP1 mRNA/protein expression. |
| Confidence score: |
0.8554 |