| Expression pattern: |
UP |
| Associated gene: |
ABCE1, SRGAP2, FSCN1, VMP1, HRNR |
| Associated microRNA: |
miR-145-5p / miR-145 |
| Biological function: |
Promotes RCC cell proliferation and likely tumor progression; knockdown inhibits proliferation. |
| Molecular mechanism: |
Acts as a miR-145-5p sponge and regulates downstream proliferation-related genes including ABCE1, SRGAP2, FSCN1, VMP1, and HRNR. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes); metastasis (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; RT-qPCR; RNA-seq; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression correlated with poor prognosis and low miR-145 levels; circ-AFF2 showed diagnostic potential for RCC with AUC 0.747. |
| Description: |
circ-AFF2 is up-regulated in RCC tissues and functions as an oncogenic circRNA. It promotes RCC cell proliferation, likely through a ceRNA mechanism in which it sponges miR-145-5p and supports expression of downstream proliferation-related genes; high expression is associated with poor prognosis and diagnostic potential. |
| Confidence score: |
0.8499 |