| Expression pattern: |
UP |
| Associated gene: |
CCL5 |
| Associated microRNA: |
- |
| Biological function: |
Promotes ESCC progression, proliferation, migration and invasiveness; suppression of circMAN1A2 inhibits ESCC cell proliferation, migration and invasion. |
| Molecular mechanism: |
circMAN1A2 promotes ESCC progression by regulating the downstream target CCL5; CCL5 overexpression reverses the inhibitory effects of circMAN1A2 knockdown. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Microarray; ELISA; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circMAN1A2 expression is associated with poor prognosis in patients with ESCC; circMAN1A2 might be a novel diagnostic biomarker, prognostic biomarker, and therapeutic target for ESCC. |
| Description: |
circMAN1A2 is up-regulated in ESCC tissues and high expression is associated with poor overall survival. Functionally, circMAN1A2 promotes ESCC cell proliferation, migration and invasion, and its knockdown suppresses tumor growth in a xenograft model. Mechanistically, the study identifies CCL5 as a downstream target through which circMAN1A2 promotes ESCC progression, suggesting diagnostic, prognostic and therapeutic potential. |
| Confidence score: |
0.8262 |