| Expression pattern: |
UP |
| Associated gene: |
TGFB2, E-cadherin, Vimentin |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Hsa_circ_0001326 restrains trophoblast cell viability, proliferation, migration, invasion, and EMT in PE. |
| Molecular mechanism: |
Hsa_circ_0001326 functions as a miRNA sponge for miR-145-5p, thereby elevating TGFB2 expression and regulating trophoblast cell viability, migration, invasion, and EMT. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); ceRNA regulation (other); TGF-beta/SMAD (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; Microarray |
| Clinical significance: |
Hsa_circ_0001326 may be a potential pre-warning or diagnostic biomarker and therapeutic target for PE. |
| Description: |
Hsa_circ_0001326 is up-regulated in PE placentas and suppresses trophoblast cell viability, proliferation, migration, invasion, and EMT. Mechanistically, it sponges miR-145-5p to elevate TGFB2 expression, forming a hsa_circ_0001326/miR-145-5p/TGFB2 axis that may contribute to PE pathogenesis and serve as a potential biomarker or therapeutic target. |
| Confidence score: |
0.7759 |