| Expression pattern: |
UP |
| Associated gene: |
HTRA1 / HtrA serine peptidase 1 / HtrA Serine Peptidase 1, E-cadherin, N-cadherin |
| Associated microRNA: |
miR-188-3p |
| Biological function: |
Restrains trophoblast cell proliferation, invasion, migration and EMT, and induces apoptosis in JEG-3 and HTR8/SVneo cells. |
| Molecular mechanism: |
circ_0001326 acts as a sponge for miR-188-3p and regulates HTRA1 through sequestering miR-188-3p. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0001326 had high diagnostic value for PE; high circ_0001326 expression was associated with higher proteinuria, systolic blood pressure and diastolic blood pressure. |
| Description: |
circ_0001326 is up-regulated in placental tissues from patients with preeclampsia and shows diagnostic value for distinguishing PE placentae from normal placentae. In trophoblast cells, circ_0001326 overexpression suppresses proliferation, invasion, migration and EMT while promoting apoptosis. Mechanistically, circ_0001326 functions through the miR-188-3p/HTRA1 axis by sponging miR-188-3p to regulate HTRA1. |
| Confidence score: |
0.7859 |