circRNA basic information
circBase ID: hsa_circ_0060937
Name: hsa_circ_CYP24A1
Synonym: Circ_0060937
Host Gene: CYP24A1
Genomic location(hg19): chr20:52779255-52782372:-
Genomic location(hg38): chr20:54162716-54165833:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung cancer tissues; adjacent normal tissues; tumor tissues; non-tumor tissues; tumor tissues from xenograft models
Cell lines:

A549; H1299; HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: LAD1, CYP24A1, PCNA, MMP9, Bax, Ki-67, BAX
Associated microRNA: miR-1304-5p
Biological function: Circ_0060937 promotes lung cancer cell proliferation, colony formation, migration, invasion, sphere formation, cell cycle progression, and in vivo tumor growth, and inhibits apoptosis.
Molecular mechanism: Circ_0060937 functions as a miRNA sponge for miR-1304-5p and positively regulates LAD1 expression through the miR-1304-5p/LAD1 axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); stemness (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

Circ_0060937 was highly expressed in lung cancer clinical tissues and may be a therapeutic target for lung cancer.
Description:

Circ_0060937 is up-regulated in lung cancer tissues and A549/H1299 lung cancer cells. It acts as an oncogenic circRNA that promotes proliferation, migration, invasion, sphere formation, cell cycle progression, and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0060937 binds miR-1304-5p and relieves miR-1304-5p-mediated repression of LAD1, thereby activating the circ_0060937/miR-1304-5p/LAD1 regulatory axis.
Confidence score:

0.7554
Other information
Title:

Circ_0060937 Contributes to the Development of Lung Cancer via Positively Regulating LAD1 Expression by Binding to miR-1304-5p.

Journal: Biochemical genetics
Published: 2023
PubMed ID: 36633773
Study type:

combined biological and clinical study
Data availability: GSE112214; GEPIA; circinteractome; targetscan; supplementary material available at https:// doi. org/ 10. 1007/ s10528-022-10322-4
Code availability: -