circRNA basic information
circBase ID: hsa_circ_0047921
Name: hsa_circ_CD226
Synonym: circRNA CD226
Host Gene: CD226
Genomic location(hg19): chr18:67562936-67563281:-
Genomic location(hg38): chr18:69895700-69896045:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum exosomes; peripheral blood samples; primary lung tumors; paired nontumor lung samples; tumor tissue
Cell lines:

HCC827; A549; H1755; H1299; BEAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HMGA2, AGO2
Associated microRNA: miR-1224-3p
Biological function: promotes NSCLC cell proliferation, viability, migration, invasion, stemness and tumor growth; circCD226 depletion suppresses these malignant properties
Molecular mechanism: ceRNA mechanism; circCD226 sponges miR-1224-3p to induce HMGA2 expression and posttranscriptionally regulate HMGA2
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circCD226 has potential sensitivity and specificity for NSCLC diagnosis; high circCD226 expression is associated with shorter survival time and may serve as a potential prognosis marker
Description:

circCD226 is up-regulated in NSCLC serum exosomes, tumor tissues and cell lines, and high expression is associated with poorer survival and diagnostic potential. Functionally, circCD226 promotes NSCLC cell viability, proliferation, migration, invasion, stemness and xenograft tumor growth. Mechanistically, it acts as a ceRNA by sponging miR-1224-3p to up-regulate HMGA2.
Confidence score:

0.8791
Other information
Title:

Identification of exosomal circRNA CD226 as a potent driver of nonsmall cell lung cancer through miR-1224-3p/high mobility group AT-hook 2 axis.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 35946568
Study type:

combined biological and clinical study
Data availability: the datasets used and analyzed during the current study are available from the corresponding author upon reasonable request; Supplement Table 1, Supplemental Digital Content 1, http://links.lww.com/ACD/A447
Code availability: -