| Expression pattern: |
UP |
| Associated gene: |
RAB1A, Ago2 |
| Associated microRNA: |
miR-139-3p |
| Biological function: |
circ_0000218 promotes CRC cell proliferation, migration, invasion, metastasis and EMT; circ_0000218 knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
circ_0000218 functions as a competing endogenous RNA by adsorbing miR-139-3p, thereby up-regulating RAB1A expression in CRC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Colony Formation Assay; BrdU; Transwell Assay; Western Blot; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
High circ_0000218 expression is linked to increased T stage and lymph node metastasis, and the authors suggest it may serve as a biomarker for evaluating CRC prognosis. |
| Description: |
circ_0000218 is up-regulated in colorectal cancer tissues and cell lines and is associated with increased T stage and lymph node metastasis. Functionally, circ_0000218 promotes CRC cell proliferation, migration, invasion, metastasis and EMT. Mechanistically, it acts through the miR-139-3p/RAB1A axis by sponging miR-139-3p and increasing RAB1A expression. |
| Confidence score: |
0.7537 |