circRNA basic information
circBase ID: -
Name: hsa_circ_MAN1A2
Synonym: circMAN1A2
Host Gene: MAN1A2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma / NPC
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Tumor tissues; adjacent normal nasopharyngeal tissues; NPC tissues; tumor xenografts; lungs; inguinal lymph nodes
Cell lines:

NP69; HK-1; C666-1; HEK293T; HEK293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: UBR5, ATMIN, Ago2
Associated microRNA: miR-135a-3p
Biological function: Promotes NPC cell proliferation, clonogenic ability, migration, invasion, EMT, tumor growth, lung metastasis, and inguinal lymph node metastasis; circMAN1A2 knockdown represses these malignant phenotypes.
Molecular mechanism: circMAN1A2 acts as a cytoplasmic ceRNA sponge for miR-135a-3p, up-regulates UBR5, and stimulates UBR5-mediated ubiquitination of ATMIN, thereby promoting malignant behaviors and EMT in NPC.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes); ubiquitination (promotes)
Detected method:
Q
B
Validation methods:

RNase R Treatment; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; Co-IP; In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circMAN1A2 expression is associated with severer lymph node metastasis and tumor staging and predicts shorter OS, DFS, and DMFS in NPC patients; it may be a potential therapeutic target and may provide a potential novel target for future diagnosis and prognosis.
Description:

circMAN1A2 is up-regulated in NPC tissues and cells and its high expression is associated with shorter OS, DFS, and DMFS. Functionally, circMAN1A2 promotes NPC proliferation, migration, invasion, EMT, tumor growth, and metastasis. Mechanistically, cytoplasmic circMAN1A2 sponges miR-135a-3p to increase UBR5 expression, leading to UBR5-mediated ubiquitination of ATMIN.
Confidence score:

0.8637
Other information
Title:

CircMAN1A2 contributes to nasopharyngeal carcinoma progression via enhancing the ubiquitination of ATMIN through miR-135a-3p/UBR5 axis.

Journal: Human cell
Published: 2023
PubMed ID: 36626032
Study type:

combined biological and clinical study
Data availability: The data underlying this article will be shared on reasonable request to the corresponding author; Supplementary material available at https:// doi. org/ 10. 1007/ s13577-022-00831-0
Code availability: -