circRNA basic information
circBase ID: hsa_circ_0005615
Name: hsa_circ_NFATC3
Synonym: circ_0005615
Host Gene: NFATC3
Genomic location(hg19): chr16:68155889-68157024:+
Genomic location(hg38): chr16:68121986-68123121:+
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; paracancerous normal tissues; blood samples; serum exosomes
Cell lines:

HCT116; LOVO; SW480; FHC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FOSL2, cyclinD1, PCNA
Associated microRNA: miR-873-5p
Biological function: Promotes CRC cell proliferation, migration, invasion, metastasis, and tumor growth; inhibits apoptosis.
Molecular mechanism: ceRNA mechanism: circ_0005615 sponges miR-873-5p to regulate FOSL2 expression through the miR-873-5p/FOSL2 signaling pathway.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0005615 expression was associated with lymph node metastasis, TNM stage, tumor size, and poor survival rate; circ_0005615 might be a novel diagnostic biomarker for CRC and a possible therapeutic target through the circ_0005615/miR-873-5p/FOSL2 axis.
Description:

circ_0005615 is up-regulated in CRC tissues, CRC cell lines, and serum exosomes from CRC patients. It promotes malignant CRC progression by sponging miR-873-5p and increasing FOSL2 expression, thereby enhancing proliferation, migration, invasion, metastasis, and tumor growth while suppressing apoptosis. High circ_0005615 expression is associated with adverse clinicopathological features and poor survival, suggesting biomarker and therapeutic relevance.
Confidence score:

0.8069
Other information
Title:

Circ_0005615 Regulates the Progression of Colorectal Cancer Through the miR-873-5p/FOSL2 Signaling Pathway.

Journal: Biochemical genetics
Published: 2023
PubMed ID: 36920708
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -