circRNA basic information
circBase ID: hsa_circ_0002980
Name: hsa_circ_AGK
Synonym: circAGK
Host Gene: AGK
Genomic location(hg19): chr7:141336759-141349133:+
Genomic location(hg38): chr7:141636959-141649333:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005339
MONDO name: androgenetic alopecia
Disease details: androgen alopecia
Disease DO ID:
 0050801
Disease MeSH ID:
 -
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

hair follicle tissue; posterior occipital hair follicles; hair follicle specimens; skin
Cell lines:

human DPCs; 293T
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: BAX
Associated microRNA: miR-3180-5p
Biological function: CircAGK promotes DPC apoptosis in a high DHT environment and inhibits hair growth in AGA mice in vivo.
Molecular mechanism: CircAGK acts through targeted binding and reverse regulation of miR-3180-5p, thereby regulating BAX and promoting DPC apoptosis via the miR-3180-5p/BAX axis.
Biological pathway or process:

apoptosis (promotes); ceRNA regulation (other); other pathway/process (inhibits)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; In Vivo Animal Model; H&E Staining; ELISA; Bioinformatics Analysis
Clinical significance:

circAGK is a potential target for the clinical treatment of AGA.
Description:

This study identifies hsa_circ_0002980/circAGK as an up-regulated circRNA in hair follicles from AGA patients. circAGK is a cytoplasmic circular RNA derived from AGK and promotes DHT-induced DPC apoptosis by regulating the miR-3180-5p/BAX axis. In vivo, circAGK overexpression inhibits hair growth in an AGA mouse model, suggesting therapeutic relevance for AGA.
Confidence score:

0.8107
Other information
Title:

CircAGK regulates high dihydrotestosterone-induced apoptosis in DPCs through the miR-3180-5p/BAX axis.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Published: 2023
PubMed ID: 36607259
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -