| Expression pattern: |
UP |
| Associated gene: |
HuR, XIRP1 |
| Associated microRNA: |
- |
| Biological function: |
promotes invasion and migration; promotes tumor aggressiveness/expansion; promotes EMT |
| Molecular mechanism: |
HIF1alpha induces circPLOD2b; circPLOD2b binds HuR and competitively inhibits HuR-XIRP1 mRNA interaction, causing XIRP1 mRNA degradation/downregulation |
| Biological pathway or process: |
migration (promotes); invasion (promotes); EMT (promotes); mRNA stability (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; FISH / smFISH; RNA Pull-Down; Western Blot; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; Actinomycin D / DRB Stability Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation; IF (Immunofluorescence) |
| Clinical significance: |
circPLOD2b is highly expressed in GBM compared with lower grade glioma and is negatively correlated with XIRP1 expression |
| Description: |
circPLOD2b (hsa_circ_0067682) is a hypoxia/HIF1alpha-induced circRNA in glioblastoma, mainly localized in the cytoplasm. It promotes invasion/migration and xenograft progression by binding HuR and competitively reducing HuR association with XIRP1 mRNA, leading to XIRP1 downregulation and enhanced EMT/aggressiveness. |
| Confidence score: |
0.8682 |