| Expression pattern: |
UP |
| Associated gene: |
PFN2, Ago2 |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
promotes proliferation, metastasis, invasion, and EMT; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
circATXN7 acts as a miR-7-5p sponge to upregulate PFN2 |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
high circATXN7 level is associated with TNM grade, lymph node metastasis, and tumor size |
| Description: |
circATXN7 is up-regulated in NSCLC tissues and cell lines and mainly localizes to the cytoplasm. It promotes NSCLC proliferation, invasion/metastasis and EMT while inhibiting apoptosis, partly by sponging miR-7-5p to de-repress PFN2; circATXN7 knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.8039 |