| Expression pattern: |
UP |
| Associated gene: |
SIRT7, p53, p21, cleaved caspase-3 |
| Associated microRNA: |
miR-3666 |
| Biological function: |
circPVT1 promotes HCC cell growth and colony formation and inhibits apoptosis; knockdown of circPVT1 restricts HCC cell growth and promotes apoptosis. |
| Molecular mechanism: |
circPVT1 functions as a sponge for miR-3666, thereby regulating SIRT7 expression and modulating p53 signaling in HCC cell growth. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other); p53 signaling (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
circPVT1 may serve as a potential therapeutic target and a novel intervention target for anti-HCC therapeutic approaches. |
| Description: |
circPVT1 is upregulated in HCC tissues and cell lines and acts as an oncogenic circRNA that promotes HCC cell growth and colony formation while suppressing apoptosis. Mechanistically, circPVT1 sponges miR-3666 to relieve repression of SIRT7, thereby contributing to HCC cell growth and modulation of p53 signaling. The study suggests circPVT1 and the circPVT1/miR-3666/SIRT7 axis as potential therapeutic targets for HCC. |
| Confidence score: |
0.7032 |