| Expression pattern: |
DS |
| Associated gene: |
IGF-1, AGO2 |
| Associated microRNA: |
miR-29a |
| Biological function: |
protects CMVECs from oxidative damage by decreasing apoptosis and oxidative stress (ROS, MDA) and increasing antioxidant capacity (SOD); improves CMVEC survival under oxidative conditions |
| Molecular mechanism: |
Exosomal circHIPK3 functions as a ceRNA/miRNA sponge for miR-29a, inhibiting miR-29a activity and thereby increasing IGF-1 expression in CMVECs under oxidative stress. |
| Biological pathway or process: |
apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; TUNEL; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; CCK8 |
| Clinical significance: |
- |
| Description: |
In hypoxia-pretreated cardiomyocyte-derived exosomes, circHIPK3 is increased and can be transferred into CMVECs. circHIPK3 protects CMVECs from H2O2-induced oxidative injury by sponging miR-29a and relieving miR-29a-mediated repression of IGF-1, reducing apoptosis and oxidative stress. |
| Confidence score: |
0.7449 |