circRNA basic information
circBase ID: mmu_circ_0002207
Name: mmu_circ_FOXO3
Synonym: CircFOXO3
Host Gene: FOXO3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005178
MONDO name: osteoarthritis
Disease details: Osteoarthritis / OA
Disease DO ID:
 8398
Disease MeSH ID:
 D010003
Disease NCIt ID:
 C3293
Disease ICD11 ID:
 558562409
Disease OMIM ID:
 -
Species: Mouse
Species details: Mus musculus
Tissue specimen:

articular cartilage / OA cartilage tissues / cartilage sections
Cell lines:

ATDC5
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: FOXO3
Associated microRNA: -
Biological function: protects against OA by increasing chondrocyte viability, inhibiting apoptosis, and maintaining ECM homeostasis; enhances autophagy
Molecular mechanism: circFOXO3 interacts with and targets its parental gene FOXO3; promotes autophagy by inhibiting PI3K/AKT signaling, thereby reducing apoptosis and ECM catabolism
Biological pathway or process:

PI3K/AKT (inhibits); autophagy (promotes); apoptosis (inhibits); proliferation (promotes); other pathway/process (other)
Detected method:
Q
H
Validation methods:

RT-qPCR; FISH / smFISH; RNase R Treatment; divergent primers PCR; Sanger Sequencing; RIP (RNA Immunoprecipitation); Transfection; CCK8; TUNEL; IF (Immunofluorescence); IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circFOXO3 is down-regulated in osteoarthritic cartilage and inflammatory (IL-1beta) conditions. It protects chondrocytes by interacting with its host gene FOXO3 and enhancing autophagy via inhibition of PI3K/AKT signaling, thereby reducing apoptosis and ECM catabolism; intra-articular lentiviral circFOXO3 alleviates OA in a DMM mouse model.
Confidence score:

0.7827
Other information
Title:

CircFOXO3 protects against osteoarthritis by targeting its parental gene FOXO3 and activating PI3K/AKT-mediated autophagy.

Journal: Cell death & disease
Published: 2022
PubMed ID: 36344492
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -