| Expression pattern: |
DN |
| Associated gene: |
PDCD4 |
| Associated microRNA: |
miR-421 |
| Biological function: |
Suppresses cell proliferation and induces apoptosis in OSCC cells; acts as a tumor suppressor |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-421, thereby relieving repression of PDCD4 |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circ-ITCH expression was strongly associated with adverse clinicopathological characteristics of OSCC patients; patients with lower circ-ITCH expression had significantly shorter overall survival |
| Description: |
circ-ITCH is down-regulated in OSCC tissues and cell lines and functions as a tumor suppressor. It mainly localizes to the cytoplasm and acts as a ceRNA to sponge miR-421, relieving miR-421-mediated repression of PDCD4, thereby inhibiting proliferation and promoting apoptosis in OSCC cells. |
| Confidence score: |
0.7325 |