circRNA basic information
circBase ID: hsa_circ_0001821
Name: hsa_circ_PVT1
Synonym: circPVT1
Host Gene: PVT1
Genomic location(hg19): chr8:128902834-128903244:+
Genomic location(hg38): chr8:127890588-127890998:+
Subcellular localization: cytoplasm; extracellular vesicle
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008433
MONDO name: small cell lung carcinoma
Disease details: small cell lung cancer / SCLC
Disease DO ID:
 5409, 5411
Disease MeSH ID:
 D055752
Disease NCIt ID:
 C4917
Disease ICD11 ID:
 1800431439
Disease OMIM ID:
 182280
Species: Human
Species details: Homo sapiens
Tissue specimen:

normal lung pooled sample
Cell lines:

GLC1DM; GLC1HSR; GLC2; GLC3; NCI-H2171; GLC26; GLC28; H1963; H510A; HCC33; H2141; H1184; GLC8; GLC14; BEAS-2B; HeLa
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MYC, BCL2, YAP1, POU2F3
Associated microRNA: -
Biological function: circPVT1 promotes lung cell proliferation, reduces apoptosis, increases MYC and BCL2 expression, modulates SCLC subtype-related master regulators by increasing YAP1 and decreasing POU2F3, and is secreted in extracellular vesicles from SCLC cell lines.
Molecular mechanism: circPVT1 overexpression induces transcriptional and protein-level changes involving MYC, BCL2, YAP1, POU2F3, proliferation factors, and apoptosis-related genes; it is unlikely to encode a protein product and can be packaged into extracellular vesicles.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); Hippo/YAP (promotes); other pathway/process (other)
Detected method:
Q
B
Validation methods:

RNase R Treatment; RT-qPCR; Northern Blot; Transfection; Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis
Clinical significance:

Potential biomarker for MYC-driven SCLC and noninvasive liquid biopsy-based approaches, based on detection in extracellular vesicles secreted by SCLC cell lines.
Description:

This study reports that circPVT1 is overexpressed in SCLC cell lines, especially MYC-amplified contexts. Functional overexpression experiments indicate that circPVT1 promotes proliferation, inhibits apoptosis, increases MYC/BCL2/YAP1, decreases POU2F3, and may contribute to SCLC tumorigenesis and subtype definition. circPVT1 is also detected in extracellular vesicles from SCLC cell lines, supporting potential biomarker relevance.
Confidence score:

0.593
Other information
Title:

circPVT1 and PVT1/AKT3 show a role in cell proliferation, apoptosis, and tumor subtype-definition in small cell lung cancer.

Journal: Genes, chromosomes & cancer
Published: 2023
PubMed ID: 36562080
Study type:

combined biological and bioinformatics study
Data availability: GSE83227; GSE60052; supplementary material
Code availability: -