| Expression pattern: |
UP |
| Associated gene: |
FASN |
| Associated microRNA: |
miR-545-3p |
| Biological function: |
Promotes pancreatic cancer cell growth, migration, invasion, EMT, tumor growth in vivo, and M2 macrophage polarization; suppresses apoptosis and cell cycle arrest. |
| Molecular mechanism: |
circ_0018909 acts as a ceRNA sponge for miR-545-3p to regulate FASN expression and promotes M2 macrophage polarization. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); cell cycle (promotes); macrophage polarization (promotes); immune regulation (promotes); lipid metabolism (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
circ_0018909 was elevated in advanced-stage pancreatic cancer tissues and pancreatic cancer-positive patients, indicating a potential biomarker for pancreatic cancer therapy. |
| Description: |
circ_0018909 is up-regulated in pancreatic cancer tissues and cells and is associated with advanced clinical stage. Functionally, circ_0018909 promotes pancreatic cancer cell proliferation, migration, invasion, EMT, tumor growth, and M2 macrophage polarization while inhibiting apoptosis. Mechanistically, it sponges miR-545-3p to increase FASN expression. |
| Confidence score: |
0.8299 |