| Expression pattern: |
UP |
| Associated gene: |
FOXO1 mRNA, FOXO1 |
| Associated microRNA: |
miR-145 |
| Biological function: |
circSHOC2 silencing repressed high glucose-induced HUVEC pyroptosis and recovered proliferation ability; up-regulated circSHOC2 participates in pyroptosis of HG-induced vascular endothelial cells. |
| Molecular mechanism: |
circSHOC2 acts as a miRNA sponge for miR-145 and regulates FOXO1 through the circSHOC2/miR-145/FOXO1 axis in high glucose-induced vascular endothelial cells. |
| Biological pathway or process: |
pyroptosis (promotes); proliferation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
providing a potential solution for diabetic angiopathy |
| Description: |
circSHOC2 is up-regulated in high glucose-induced HUVECs and contributes to vascular endothelial cell pyroptosis. Silencing circSHOC2 restores proliferation and reduces pyroptosis markers and inflammatory cytokines. Mechanistically, circSHOC2 functions as a miR-145 sponge to regulate FOXO1 through the circSHOC2/miR-145/FOXO1 axis. |
| Confidence score: |
0.7884 |