| Expression pattern: |
UP |
| Associated gene: |
PRKAA2, AGO2 |
| Associated microRNA: |
miR-21-3p |
| Biological function: |
promotes 5-FU chemoresistance and autophagy; inhibits apoptosis |
| Molecular mechanism: |
Acts as a miRNA sponge: circCPM directly binds miR-21-3p in the cytoplasm to upregulate PRKAA2, activating autophagy and promoting 5-FU resistance. |
| Biological pathway or process: |
autophagy (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; divergent primers PCR; RT-qPCR; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); Transmission electron microscopy (TEM); In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
High circCPM expression is positively associated with poor survival in gastric cancer patients receiving 5-FU-based chemotherapy; circCPM could be a biomarker for 5-FU resistance. |
| Description: |
circCPM (circ0027497), derived from CPM, is up-regulated in 5-FU-resistant gastric cancer tissues/cells. It promotes 5-FU chemoresistance by sponging miR-21-3p in the cytoplasm, thereby increasing PRKAA2 expression and activating autophagy while reducing apoptosis, and its high expression correlates with poor survival. |
| Confidence score: |
0.8912 |