| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha |
| Associated microRNA: |
miR-1272 |
| Biological function: |
promotes CRC cell viability/proliferation, colony formation, and glycolysis (Warburg effect) under hypoxia |
| Molecular mechanism: |
HIF-1alpha binds circ_0006508 promoters to enhance its transcription under hypoxia; circ_0006508 acts as a ceRNA to sponge miR-1272, relieving miR-1272 suppressive effects on glycolysis |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circ_0006508 expression is associated with advanced TNM stage and predicts worse overall survival in CRC patients. |
| Description: |
In CRC, circ_0006508 is up-regulated and induced by hypoxia via HIF-1alpha-driven transcription. It mainly localizes to the cytoplasm and promotes proliferation/colony formation and the Warburg effect by sponging miR-1272. High circ_0006508 expression correlates with advanced TNM stage and poorer overall survival. |
| Confidence score: |
0.725 |