circRNA basic information
circBase ID: -
Name: hsa_circ_DNA2
Synonym: circ_002363
Host Gene: DNA2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005275
MONDO name: lung disorder
Disease details: pulmonary toxicity
Disease DO ID:
 850
Disease MeSH ID:
 D008171
Disease NCIt ID:
 C3198
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

rat lung tissue; rat serum; peripheral blood
Cell lines:

16HBE
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: PARP1, Ku70, Ku80, Rad50
Associated microRNA: -
Biological function: Suppresses NPs-Nd 2 O 3-induced DNA damage in 16HBE cells and may serve as a clinical marker for occupational workers in early pulmonary disease.
Molecular mechanism: Circ_002363 binds PARP1, Ku70, Ku80 and Rad50 and inhibits DNA damage by regulating the NHEJ DNA repair pathway; its downregulation after NPs-Nd 2 O 3 exposure is caused by BCAS2 interacting with Pre-DNA2 and affecting circ_002363 cyclization.
Biological pathway or process:

other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

Circ_002363 is a potential clinical marker for the diagnosis of occupational workers in early pulmonary disease; longer working years were associated with lower expression of circ_002363.
Description:

Circ_002363 is downregulated after NPs-Nd 2 O 3 exposure in 16HBE cells, exposed rats and occupational workers. It suppresses nanoparticle-induced DNA damage by promoting the NHEJ repair pathway through interactions with PARP1, Ku70, Ku80 and Rad50, while BCAS2-mediated regulation of Pre-DNA2 contributes to its reduced formation.
Confidence score:

0.8635
Other information
Title:

Circ_002363 is regulated by the RNA binding protein BCAS2 and inhibits neodymium oxide nanoparticle-induced DNA damage by non-homologous end-joining repair.

Journal: The Science of the total environment
Published: 2023
PubMed ID: 36526188
Study type:

combined biological and clinical study
Data availability: Supplementary data; https://doi.org/10.1016/j.scitotenv.2022.160819
Code availability: -