| Expression pattern: |
DN |
| Associated gene: |
SHPRH-146aa, SHPRH, PCNA, DTL |
| Associated microRNA: |
- |
| Biological function: |
circ-SHPRH encodes SHPRH-146aa, which acts as a tumor suppressor, reduces malignant behavior and tumorigenicity, and inhibits cell proliferation in glioblastoma. |
| Molecular mechanism: |
circ-SHPRH uses overlapping genetic codes and an IRES-associated ORF to translate SHPRH-146aa; SHPRH-146aa protects full-length SHPRH from ubiquitin-proteasome degradation, enabling SHPRH-mediated ubiquitination of PCNA and suppression of proliferation and tumorigenicity. |
| Biological pathway or process: |
proliferation (inhibits); ubiquitination (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
B
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Northern Blot; RT-qPCR; RNA-seq; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
SHPRH-146aa expression is associated with prognosis in glioblastoma; patients with higher SHPRH-146aa or SHPRH expression had longer survival time. |
| Description: |
circ-SHPRH is a human SHPRH-derived circular RNA that is down-regulated in glioblastoma. It encodes the 17 kDa protein SHPRH-146aa, which suppresses glioblastoma cell proliferation and tumorigenicity by protecting full-length SHPRH from ubiquitin-proteasome degradation and promoting SHPRH-mediated PCNA ubiquitination. Higher expression of the encoded SHPRH-146aa is associated with longer survival in glioblastoma patients. |
| Confidence score: |
0.7886 |