| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, IGF2BP1, IGF2BP2 |
| Associated microRNA: |
miR-370 |
| Biological function: |
promotes migration and invasion; promotes metastasis |
| Molecular mechanism: |
circFOXK2 physically interacts with IGF2BP3 and miR-370 to synergistically promote breast cancer metastasis; circFOXK2 increases miR-370 and promotes transfer of exosomal miR-370 to recipient cells |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Transfection; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; circRIP |
| Clinical significance: |
upregulation of circFOXK2 was correlated with poor clinicopathological characteristics; correlated with invasive histological type, lymph node metastasis, and advanced stage; potential biomarker for diagnosis and treatment |
| Description: |
circFOXK2 (hsa_circ_0000816) is up-regulated in highly metastatic breast cancer cells and advanced-stage/metastatic tissues. It promotes migration, invasion, and in vivo metastasis, and mechanistically cooperates with the RBP IGF2BP3 and miR-370; circFOXK2 increases miR-370 and enhances exosomal miR-370 transfer to recipient cells to boost metastatic ability. |
| Confidence score: |
0.7618 |