| Expression pattern: |
UN |
| Associated gene: |
EML4-ALK1, ALK, PI3K, AKT, mTOR, JAK3, STAT3, MEK1/2, ERK1/2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes cell proliferation, migration and invasion; regulates cell cycle; inhibits apoptosis; increases drug sensitivity to crizotinib when knocked down; promotes xenograft tumor growth. |
| Molecular mechanism: |
Positively regulates the parental fusion gene EML4-ALK1, thereby activating ALK downstream signaling at the protein level (PI3K-AKT-mTOR; JAK3-STAT3; MEK-ERK1/2). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); PI3K/AKT/mTOR (promotes); JAK/STAT (promotes); ERK (promotes); drug resistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
F-circEA1 is a fusion circRNA derived from the EML4-ALK1 fusion gene in NSCLC cells. It promotes proliferation, migration/invasion and tumor growth, inhibits apoptosis, and modulates crizotinib response, at least partly by upregulating EML4-ALK1 and activating ALK downstream signaling (PI3K/AKT/mTOR, JAK3/STAT3, MEK/ERK). |
| Confidence score: |
0.7633 |