circRNA basic information
circBase ID: hsa_circ_0000943
Name: hsa_circ_ARHGAP35
Synonym: -
Host Gene: ARHGAP35
Genomic location(hg19): chr19:47421744-47440665:+
Genomic location(hg38): chr19:46918487-46937408:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

fresh GBM tissue; fresh para-tumor tissue / peritumoral edema tissue containing GBM cells
Cell lines:

LN229; T98G; HEB
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: ARHGAP35, HIF-1alpha-centered predicted mRNA network
Associated microRNA: -
Biological function: May play a role in GBM cell malignant phenotype under hypoxic condition; no individual functional knockdown/overexpression assay was reported for this circRNA.
Molecular mechanism: Predicted HIF-1alpha-centered circRNA-miRNA-mRNA network, with ARHGAP35 indicated as the target gene.
Biological pathway or process:

ceRNA regulation (other); HIF-1 signaling (other); migration (other); invasion (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

Potential candidate for prognostic markers and adjuvant therapeutics of GBM in the future.
Description:

hsa_circ_0000943 is down-regulated in hypoxic GBM cell lines and in GBM tissues. The authors linked it to ARHGAP35 and suggested that it may participate in GBM malignant phenotypes under hypoxia, though its specific mechanism was not experimentally validated.
Confidence score:

0.5994
Other information
Title:

Profiling and Bioinformatics Analyses of Differential Circular RNA Expression in Glioblastoma Multiforme Cells Under Hypoxia.

Journal: Journal of molecular neuroscience : MN
Published: 2022
PubMed ID: 36484975
Study type:

combined biological, clinical and bioinformatics study
Data availability: Raw data available from the authors without undue reservation; Supplementary information at https://doi.org/10.1007/s12031-022-02090-y
Code availability: -