circRNA basic information
circBase ID: hsa_circ_0000745
Name: hsa_circ_SPECC1
Synonym: circ-SPECC1
Host Gene: SPECC1
Genomic location(hg19): chr17:20107645-20109225:+
Genomic location(hg38): chr17:20204332-20205912:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

fresh GBM tissue; fresh para-tumor tissue / peritumoral edema tissue containing GBM cells
Cell lines:

LN229; T98G; HEB
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: HIF-1alpha-centered predicted mRNA network
Associated microRNA: -
Biological function: Promotes GBM cell proliferation, migration, and invasion under hypoxia; may participate in GBM development and progression when hypoxia occurs.
Molecular mechanism: Predicted HIF-1alpha-centered circRNA-miRNA-mRNA network; definite mechanism not determined.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other); HIF-1 signaling (other); TGF-beta/SMAD (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay
Clinical significance:

Potential candidate for prognostic markers and adjuvant therapeutics of GBM in the future.
Description:

hsa_circ_0000745/circ-SPECC1 is up-regulated in hypoxic GBM cell lines and GBM tissues. Its knockdown suppresses GBM cell proliferation, migration, and invasion under hypoxia, suggesting a tumor-promoting role and potential value as a prognostic or therapeutic candidate.
Confidence score:

0.7378
Other information
Title:

Profiling and Bioinformatics Analyses of Differential Circular RNA Expression in Glioblastoma Multiforme Cells Under Hypoxia.

Journal: Journal of molecular neuroscience : MN
Published: 2022
PubMed ID: 36484975
Study type:

combined biological, clinical and bioinformatics study
Data availability: Raw data available from the authors without undue reservation; Supplementary information at https://doi.org/10.1007/s12031-022-02090-y
Code availability: -