| Expression pattern: |
UP |
| Associated gene: |
MELK |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
Promotes CRC cell proliferation and tumor formation; inhibits apoptosis; circ_0007031 knockdown represses proliferation, induces G0/G1 arrest and apoptosis, and hinders tumor growth in vivo. |
| Molecular mechanism: |
circ_0007031 acts as a sponge for miR-485-3p and upregulates MELK expression in a miR-485-3p-dependent manner. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circ_0007031 was suggested as a potential therapeutic target for CRC. |
| Description: |
circ_0007031 is up-regulated in colorectal cancer tissues and CRC cell lines. It promotes CRC malignant progression by sponging miR-485-3p to increase MELK expression, thereby enhancing proliferation and tumor formation while suppressing apoptosis. Its knockdown inhibits tumor growth in vivo, supporting circ_0007031 as a potential therapeutic target for CRC. |
| Confidence score: |
0.742 |