| Expression pattern: |
UP |
| Associated gene: |
PCOLCE, BTF3 |
| Associated microRNA: |
- |
| Biological function: |
promotes TGF-beta1-induced fibroblast activation into matrix-secreting cells and promotes fibroblast proliferation, collectively enhancing wound repair and wound closure |
| Molecular mechanism: |
CircGLIS3(2) RNA binds and stabilizes PCOLCE protein to support TGF-beta1 signaling; CircGLIS3(2)-encoded protein binds and stabilizes BTF3 to regulate cell cycle/proliferation |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); cell cycle (promotes); proliferation (promotes); fibrosis (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; IF (Immunofluorescence); Transfection; Luciferase Reporter Assay; Western Blot; Cell Cycle Assay; MTT; In Vivo Animal Model; H&E Staining; Microarray; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
CircGLIS3(2) is overexpressed in keloids compared to donor-matched normal skin; proposed as a therapeutic target for wound repair and scar reduction |
| Description: |
CircGLIS3(2) is up-regulated in dermal fibroblasts during acute human wound healing and is overexpressed in keloids. Its RNA form promotes TGF-beta1-driven fibroblast activation/ECM production by binding and stabilizing PCOLCE, while its encoded protein promotes cell-cycle progression and proliferation by binding and stabilizing BTF3, together supporting wound closure. |
| Confidence score: |
0.8588 |