circRNA basic information
circBase ID: -
Name: hsa_circ_ZNF292
Synonym: circRNA ZNF292 / cZNF292
Host Gene: ZNF292
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

SMMC7721
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SOX9, beta-catenin, CyclinD1, c-Myc, p21, Twist1, VE-cadherin, ATM, DNA-PKcs, Wnt/beta-catenin pathway
Associated microRNA: -
Biological function: cZNF292 promotes hypoxic hepatoma cell proliferation, vasculogenic mimicry and radioresistance; knockdown of cZNF292 suppresses proliferation and vasculogenic mimicry, induces G1 arrest, reduces DNA damage repair capacity, and enhances radiosensitivity.
Molecular mechanism: Hypoxia induces cZNF292 expression independently of HIF-1alpha; cytoplasmic cZNF292 binds SOX9 and inhibits SOX9 nuclear translocation, thereby activating Wnt/beta-catenin signaling and promoting proliferation, vasculogenic mimicry, DNA repair and radioresistance.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); cell cycle (promotes); angiogenesis (promotes); radioresistance (promotes); mRNA stability (other)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Tube Formation Assay; Western Blot; Colony Formation Assay; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); RNA Pull-Down; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL
Clinical significance:

cZNF292 may provide a valuable target for radiation therapy for hepatoma.
Description:

In hypoxic human hepatoma SMMC7721 cells, cZNF292 is induced and acts as a pro-tumor circRNA that supports proliferation, vasculogenic mimicry and radioresistance. Mechanistically, cytoplasmic cZNF292 binds SOX9, limits SOX9 nuclear translocation, and activates Wnt/beta-catenin signaling; cZNF292 knockdown suppresses these effects and improves radiosensitivity in vitro and in xenograft models.
Confidence score:

0.723
Other information
Title:

Knockdown of cZNF292 suppressed hypoxic human hepatoma SMMC7721 cell proliferation, vasculogenic mimicry, and radioresistance.

Journal: Cellular signalling
Published: 2019
PubMed ID: 31028816
Study type:

biological research
Data availability: Supplementary data; https://doi.org/10.1016/j.cellsig.2019.04.011
Code availability: -