| Expression pattern: |
UP |
| Associated gene: |
HMGA1, AGO2 |
| Associated microRNA: |
miR-1178-3p |
| Biological function: |
Promotes NSCLC cell proliferation, migration/metastasis and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0048764 acts as a miRNA sponge for miR-1178-3p, thereby enhancing HMGA1 expression and promoting NSCLC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Overexpression of circ_0048764 is associated with poorer overall survival and clinicopathological features including advanced TNM stage, lymphatic metastasis, moderate to poor differentiation, and tumor size of 3 cm or greater. |
| Description: |
hsa_circ_0048764/circ_0048764 is up-regulated in NSCLC tissues and cell lines and its high expression is associated with poorer overall survival. Functionally, circ_0048764 promotes NSCLC proliferation, migration/metastasis and xenograft tumor growth while suppressing apoptosis. Mechanistically, it sponges miR-1178-3p to enhance HMGA1 expression, forming a circ_0048764/miR-1178-3p/HMGA1 regulatory axis. |
| Confidence score: |
0.8298 |