| Expression pattern: |
UP |
| Associated gene: |
SPIN1, C-myc, MMP2 |
| Associated microRNA: |
miR-876-5p |
| Biological function: |
Promotes esophageal cancer cell proliferation and invasion, inhibits apoptosis, promotes radioresistance, and supports tumor growth in vivo. |
| Molecular mechanism: |
circ_0001686 acts as a ceRNA sponge for miR-876-5p to regulate SPIN1 expression through the circ_0001686/miR-876-5p/SPIN1 axis. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); radioresistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Colony Formation Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
High circ_0001686 expression is associated with lower overall survival, and circ_0001686 is proposed as a therapeutic target for improving prognosis of esophagus cancer patients. |
| Description: |
circ_0001686 is up-regulated in esophageal cancer tissues and cells and is further elevated after irradiation. It promotes malignant phenotypes and radioresistance by sponging miR-876-5p to up-regulate SPIN1, while circ_0001686 knockdown suppresses tumor growth and enhances radiosensitivity in vitro and in vivo. High circ_0001686 expression is associated with worse overall survival. |
| Confidence score: |
0.8071 |